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KMID : 0895820200300040017
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Journal of Oriental Rehabilitation Medicine
2020 Volume.30 No. 4 p.17 ~ p.30
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Healing Effect of Yukmijihwang-tang on Fracture Factor and Morphological Changes in Femur Fractured Mice
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Kim Hyun-Seok
Jeon Dong-Hwi
Oh Min-Seok
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Abstract
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Objectives: The purpose of this study is to evaluate the healing effect of Yukmijihwang- tang (YM) on femur fractured mice.
Methods: Mice were randomly divided into 6 groups: normal, control, positive control, YM with low, medium, high dosage each. All groups were prepared with femur fracture and treated diffrently. In order to measure bone regeneration effects, we analysed the levels of cyclooxygenase-2 (COX2), bone morphogenetic protein-2 (BMP2), collagen type Ⅱ alpha 1 chain (Col2a1), Sox9, runt-related transcription factor 2 (Runx2), and osterix genes expressed in bone. For morphological analysis, muscles were removed and femur was observed with naked eye.
Results: COX2 gene expression in bone marrow significantly decreased. BMP2 gene expression significantly increased. Col2a1 gene expression significantly increased. Sox9 gene expression increased as well. Runx2 gene expression in bone marrow increased, but there was no statistical significance. Osterix gene expression significantly increased. Union of the fracture site progressed more in YM group compared to the control group. The fracture union score was significantly decreased in YM group compared to the control group.
Conclusions: YM showed anti-inflammatory effect, promoted bone regeneration by stimulating the bone regeneration factor. In conclusion, YM can help fracture healing and it well be applied clinically to patients with fracture.
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KEYWORD
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Korean traditional medicine, Anti-inflammatory agents, Gene expression, Bone regeneration, Fracture healing
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